Journal: Cells
Article Title: E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster
doi: 10.3390/cells13181535
Figure Lengend Snippet: Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.
Article Snippet: The other antibodies used for Western blotting were monoclonal antibodies, including rabbit monoclonal antibodies against PCNA (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit monoclonal antibodies against Cyclin B1 (1:1000, ABclonal, Wuhan, China), rabbit monoclonal antibodies against CDK2 (1:1000, Cell Signaling Technology, Boston, MA, USA), mouse monoclonal antibodies against Histone H3 (1:5000, Proteintech, Chicago, IL, USA), and HRP-conjugated rabbit monoclonal against beta-Tubulin (1:10,000, Proteintech, Chicago, IL, USA).
Techniques: Flow Cytometry, Labeling, Two Tailed Test, Isolation, Western Blot, Standard Deviation, Expressing, Control
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